OUR SCIENCE
Stem cell therapies have the potential to do for chronic diseases what antibiotics did for infectious diseases.
HOW Dezawa MuseCells®
Are extracted
Dezawa MuseCells® naturally exist in very small numbers within adult tissues such as bone marrow, blood, fat, and skin. To make use of them, these rare cells must be carefully identified and purified with specific markers and techniques.
At MCI, we provide licenses for the patented extraction process designed for this purpose. All research findings and clinical results are based on cells produced with this exact method and cannot be assumed to apply to cells prepared in other ways.
Because the process is standardized, it also ensures replicability: when other laboratories use the same licensed approach, the results are consistent.
1. Source Identification:
COMMON SOURCES INCLUDE:
- Bone marrow
- Adipose tissue
- Skin fibroblasts
- Peripheral blood
2. Isolation
- SSEA-3 Marker: MUSE cells are identified by their expression SSEA-3 marker, a surface antigen. This is critical because most traditional mesenchymal stem cells do not express this marker.
REQUIRED ANTIBODIES TO CHECK FOR SSEA3
BioLegend (330302) Purified anti-human/mouse SSEA-3 (clone MC-631)Thermo Fisher Scientific (MA1-020) SSEA3 Monoclonal Antibody (MC-631)
R&D Systems (MAB1434) Human/Mouse SSEA-3 Monoclonal Antibody (clone MC-631)
Abcam (ab16286) Anti-SSEA3 antibody [MC631]
Stemcell Technologies (60061.1) Anti-Mouse SSEA-3 Antibody, Clone MC-631
- Flow Cytometry or Magnetic Sorting: Advanced laboratory techniques like fluorescence-activated cell Sorting (FACS) or magnetic-activa.
3. Culture and expansion
Culture and Expansion: Once isolated, Dezawa MuseCells® are cultured under highly specific, patented conditions designed to promote growth while preserving their natural characteristics. Unlike other stem cells, Dezawa MuseCells® do not require feeder layers, viral reprogramming, or artificial differentiation protocols.
Every ingredient and step in this process matters: even small variations in preparation can alter cell behavior and safety. That is why all published research and clinical results apply only to Dezawa MuseCells® manufactured under MCI’s licensed methods.
All published information utilizes Dezawa MuseCells® that are manufactured in accordance with MCIs patented processes.
HOW MUSE STEMS
Differ from traditional MSC stem cells
Dezawa MuseCells® and their exosomes have been studied primarily in Japan and, in published research, have demonstrated advantages over traditional MSCs and their exosomes — including pluripotency, stress resistance, and a favorable safety profile. These findings apply only to cells produced under Muse Cell Innovations®–licensed methods; products manufactured differently are not supported by this research.